Introduction. Microfabrication offers opportunities to study surface concepts focused to reduce bacterial adhesion on implants\nusing human minimally invasive rapid screening (hMIRS). Wide information is available about cell/biomaterial interactions using\neukaryotic and prokaryotic cells on surfaces of dental materials with different topographies, but studies using human being are still\nlimited. Objective. To evaluate a synergy of microfabrication and hMIRS to study the bacterial adhesion on micropatterned\nsurfaces for dental materials. Materials and Methods. Micropatterned and flat surfaces on biomedical PDMS disks were produced\nby soft lithography. The hMIRS approach was used to evaluate the total oral bacterial adhesion on PDMS surfaces placed in the\noral cavity of five volunteers (the study was approved by the University Ethical Committee). After 24 h, the disks were analyzed\nusing MTT assay and light microscopy. Results. In the present pilot study, microwell structures were microfabricated on the\nPDMS surface via soft lithography with a spacing of 5 �¼m. Overall, bacterial adhesion did not significantly differ between the flat\nand micropatterned surfaces. However, individual analysis of two subjects showed greater bacterial adhesion on the micropatterned\nsurfaces than on the flat surfaces. Significance. Microfabrication and hMIRS might be implemented to study the\ncell/biomaterial interactions for dental materials.
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